effect of Streptococccus lactis bacteriophage c2 DNA on the growth of Bacillus cereus by Gerry Charles Anderson

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  • Bacteriophages.,
  • DNA.

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Statementby Gerry Charles Anderson.
The Physical Object
Pagination[12], 81 leaves, bound :
Number of Pages81
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Open LibraryOL14259214M

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The effect of Streptococccus lactis bacteriophage c2 DNA on the growth of Bacillus cereus Public Deposited. Analytics × Add to Author: Gerry Charles Anderson.

The effect of Streptococccus lactis bacteriophage c2 DNA on the growth of Bacillus cereus. Title: The Effect of Streptococcus lactis Bacteriophage.

c2 DNA on the Growth of Bacillus cereus. Abstract approved: Dorothy K. Fraser. A lysate of Streptococcus lactis phage c2 was capable of kill­ ing 95 percent of the vegetative cells of Bacillus cereus R, as determined by plate count. Detailed studies were undertaken of this phenomenon. Bacillus thuringiensis is an important entomopathogenic bacterium belongs to the Bacillus cereus group, which also includes B.

anthracis and B. l genomes of phages originating from this group had been sequenced, but no genome of Siphoviridae phage from B. thuringiensis has been reported. We recently sequenced and analyzed the genome of a novel phage, BtCS33, from a B Cited by: This study aimed to evaluate the possible inhibitory effect of natural lactic acid bacteria on the growth of 2 Bacillus cereus strains.

First, we evaluated the behavior of spores of B. cereus GPe2 and D43 when inoculated before cheesemaking using pasteurized or raw milk; no statistical differences were observed between cheese produced with the.

GENOME ANNOUNCEMENT. Bacillus cereus is a soil bacterium and is generally propagated to vegetables and foods (4, 6, 7).It sometimes causes severe food poisoning with symptoms such as nausea, vomiting, and diarrhea (4, 13).Because bacteriophage treatment was allowed for use in foods by the U.S.

FDA (), it would be useful to ensure food safety from B. cereus contamination (9, 14).Cited by: Bacillus cereus is a ubiquitous spore-forming organism that is infrequently implicated in extraintestinal infections.

The authors report three cases of B cereus bacteremia among injection drug users presenting within one month to an urban tertiary care hospital.

Treatment with intravenous vancomycin was successful in all three cases. While temporal association suggested an outbreak, molecular.

Bacterial strains and growth conditions. The nonemetic type strain ATCC of Bacillus cereus was used in this study.

It was propagated in TrB broth (10 g/liter tryptone, 5 g/liter NaCl), TrB solidified with % (swarm plates) or 2% (nonswarm plates) (wt/vol) Difco granulated agar (Becton Dickinson, Franklin Lakes, NJ), Mueller Hinton broth (MH) (Oxoid, Hampshire, United Kingdom), or Cited by:   2.

The B. cereus Group and Its Taxonomic Issues. According to the current taxonomy, the B. cereus group includes seven recognized species: B. anthracis, B.

cereus, B. thuringiensis, Bacillus weihenstephanensis, Bacillus mycoides, Bacillus pseudomycoides and Bacillus cytotoxicus, which share a close genetic and biochemical kinship [19,20].As it was previously mentioned, the three Cited by: Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndromes1.

It is closely related to the animal and human pathogen Bacillus anthracis and the Cited by: inhibit the growth of a least one Lactobacillus strain. Putative phages that are defective or have not been shown to be infectious, such as phage -like particles, are not listed.

The Bacillus cereus group, also known as B. cereus sensu lato, consists of Gram-positive, rod-shaped, spore-forming aerobic bacteria that are widespread in Cited by:   Bacillus cereus causes food poisoning, resulting in vomiting and diarrhea, due to production of enterotoxins.

As a means of controlling this food-borne pathogen, the virulent bacteriophage B4 was isolated and characterized. Bacterial challenge assays showed that phage B4 effectively inhibited growth of members of the B.

cereus group as well as B. subtilis, and growth Cited by: Streptococcus is a genus of Gram-positive coccus (plural cocci) or spherical bacteria that belongs to the family Streptococcaceae, within the order Lactobacillales (lactic acid bacteria), in the phylum Firmicutes.

Cell division in streptococci occurs along a single axis, so as they grow, they tend to form pairs or chains that may appear bent or twisted. (Contrast with that of staphylococci Class: Bacilli. 1 CHAPTER ONE INTRODUCTION Bacillus cereus is spore forming, aerobic to facultative, gram-positive, motile rod, ubiquitously opportunistic pathogen, frequently isolated from soil and growing plants, but it is also well adapted for growth in the intestinal tract of insects and mammals.

MORPHOLOGICAL CHARACTERISTICS B. cereus is a Gram. Bacillus cereus is mainly known for causing food poisoning and severe eye infections, but it is also an opportunistic human pathogen, causing different local and systemic infections. In these cases, there is still poor recognition of the various mechanisms ruled by B.

cereus in the pathogenesis of the human diseases. In early stationary phase, B. cereus produces several toxins, such as Cited by: 2. Nucleotide (GenBank): AY Bacillus cereus spore germination receptor operon, complete sequence.

Nucleotide (GenBank): ACLT Bacillus cereus ATCCwhole genome shotgun sequencing project. Summary SUMMARY The Bacillus cereus group comprises the genetically closely related species Bacillus anthracis, B. cereusBacillus thuringiensis, Bacillus weihenstephanensis and Bacillus mycoides, which can cause quite different forms of this study, a rapid method for the phenotypic characterization and differentiation of this group of endospore forming.

LysA protein sequence analysis. LysA is amino acids long and has a calculated Mw of kDa and a pI of When compared to databases using BlastP, LysA shows greatest homology to endolysins and putative endolysins from bacteriophages or prophage remnants of Lactobacillus and Lactococcus ().The only protein which has significant overall similarity to LysA and Cited by: Bacillus cereus is the main factor in food poisoning resulting from toxin production.

Beside the well-known pathogenic Bacillus species, recent studies have detected the production of enterotoxins and emetic toxin by B. subtilis, B. pumilus, and B. licheniformis, resulting in food-borne illnesses [ 17 – 19 ].Cited by: Bacteriophage of Bacillus cereus-anthracis, DDBa PTA frozen For-Profit: $ Non-Profit: $ Summary.

Although cattle mastitis is mostly due to staphylococci, streptococci, and Escherichia coli, Bacillus cereus may behave as an agent of mammary gland infection in cows and goats.

Also, Bacillus species outside the B. cereus group seem to be potential pathogens for the mammalian udder, although many cases are merely reported as related to Bacillus spp., : Vincenzo Savini.

Using plasmid vector pIL and a recently described high-efficiency transformation protocol, we performed a direct shotgun cloning experiment in Streptococcus lactis. The cloned DNA was a Bacteriophages of food-contaminating or pathogenic bacteria or the lysins thereof are used to kill such bacteria.

Examples include lysins from bacteriophages of Listeria monocytogenes and Clostridium tyrobutyricum. Tests for bacterial contamination can be made specific for specific bacteria by using the appropriate bacteriophage or lysin thereof and determining whether cells are lysed by: Bacteriophage-mediated transfer of DNA from an infected cell to a recipient bacterium is called transduction and is one of the three major mechanisms of horizontal gene transfer (HGT), alongside.

Tarek F. El-Arabi, Mansel W. Griffiths, in Foodborne Infections and Intoxications (Fourth Edition), Bacillus cereus is a gram-positive, rod-shaped, spore-forming bacterium that has the ability to grow at a variety of temperatures and pH.

Two types of illnesses arise as a result of consumption of food contaminated with B. cereus, emetic and diarrheal. Biochemical Test and Identification of Bacillus cereus. It is a Gram positive, rod-shaped, aerobic, motile, beta hemolytic bacterium found in soil and food.

Bacillus virus phi29 (Φ29 phage) is a species of virus that belongs to the bacteriophage order Caudovirales which also includes phages PZA, Φ15, BS32, B, M2Y (M2), Nf, and GA These phages, which form part of the Podoviridae family, are the smallest Bacillus phages isolated to date and are among the smallest known dsDNA phages.

See also. Φ29 DNA polymeraseOrder: Caudovirales. The sequence of the genome of the first alkaliphilic bacteriophage has been determined.

Temperate phage BCJA1 possesses a terminally redundant genome of approximately 41 kb, with a mol% G + C. Bacillus cereus is a Gram-positive, rod-shaped, facultatively anaerobic, motile, beta-hemolytic, spore forming bacterium commonly found in soil and food. The specific name, cereus, meaning "waxy" in Latin, refers to the appearance of colonies grown on blood strains are harmful to humans and cause foodborne illness, while other strains can be beneficial as probiotics for : Bacilli.

Comparatively little information is available on members of the Myoviridae infecting low G+C content, Gram-positive host bacteria of the family Firmicutes. While numerous Bacillus phages have been isolated up till now only very few Bacillus cereus phages have been characterized in detail.

Here we present data on the large, virulent, broad-host-range B. cereus phage vB_BceM_Bcv3 Cited by: Bacillus cereus PCR Detection Kit Product Insert Product # Bacillus cereus is a rod-shaped, gram-positive bacterium.

Some strains of B. cereus cause food-poisoning and other diseases such as keratitis. cereus grows at a wide range of temperature, from 4oC to 37 oC. In fact, many foodborne illnesses caused by B.

cereus are a consequence File Size: KB. A Bacillus phage is a member of a group of bacteriophages known to have bacteria in the genus Bacillus as host species. These bacteriophages have been found to belong to the families Myoviridae, Siphoviridae, Podoviridae or Tectiviridae.

Applications. The DNA polymerase of Bacillus phage phi29 is a unique and efficient polymerase with proofreading activity. Bacillus species though to be widespread in the environment, major source of food spoilage, infection is associated with feeding silage and moldy hay bacillus licheniformis Bacillus species that effect all mammals including humans, ruminants are highly susceptible and develop rapid fatal septicemia.

Table of Contents hide 1) Biochemical Test of Bacillus cereus ) Fermentation of ) Enzymatic Reactions ) Biochemical Test of Bacillus cereus ) Related Notes: Share the article on:Biochemical Test of Bacillus cereus ­Basic Characteristics Properties (Bacillus cereus) Catalase Positive (+ve) Citrate Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Positive (+ve) Growth.

If you ever thought you had the hour flu, you may have actually had a foodborne illness caused by the bacteria Bacillus cereus. Foods that have not been properly stored at safe cold or hot temperatures are the main sources of the pathogen.

Cold foods should be kept cold (below 40 degrees F), even during food service, and hot foods should be hot, which means above degrees F.

Proper. The bacteriophage lysis cassette, which comprises a lysin and a holin gene, was analyzed in 18 Lactococcus lactis phages. A muramidase motif was found in the lysins of c2-like phages, while an.

Bacillus cereus is one of the pathogens causing nosocomial bloodstream infections (BSIs). However, few reports have documented the antimicrobial susceptibility and clinical characteristics of Bacillus cereus BSI and the importance of empirical therapy. The aim of this study was to investigate the clinical characteristics and antimicrobial susceptibility of B.

cereus isolates from patients with Cited by: Abbasiliasi et al. () reported the ability of a crude extract from Lactobacillus paracasei LA07 isolated from budu to inhibit the growth of indicator microorganisms (Bacillus cereus, Lactococcus lactis, S.

aureus, Salmonella enterica, L. monocytogenes, and E. coli). The antimicrobial activity was completely inactivated when the crude. Bacillus cereus is a Gram-positive, facultatively aerobic sporeformer whose cells are large rods. Two different clinical syndromes appear to be associated with B.

cereus food poisoning, which correspond to two different toxins produced by the bacteria. Bacillus cereus is a causative agent in both gastrointestinal and in nongastrointestinal infections. Enterotoxins, emetic toxin (cereulide), hemolysins, and phoshpolipase C as well as many enzymes such as beta-lactamases, proteases and collagenases are known as potential virulence factors of B.

cereus.A special surface structure of B. cereus cells, the S-layer, has a significant role in the Cited by: outermost layer of spores of Bacillus cereus. Bacillus is associated with proteases which causes. off flavors in milk. Psychotrophic strains of bacillus. can grow while milk is refrigerated.

Diarrheal syndrome associated with bacillus. are found in proteinaceous foods. Problem foods for Bacillus are.Bacillus cereus MLST Databases. This site uses two linked databases powered by the BIGSdb genomics sequence definition database contains allele sequence and MLST profile definitions whereas the isolate database contains provenance and epidemiological information.

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